COBRApy - Constraint-Based Reconstruction and Analysis When to use Use for loading/building metabolic networks, optimizing their steady-state fluxes, knockout screens, medium design, feasible-space sampling, and gap-filling hypotheses. FBA is a constraint-based prediction; it does not infer kinetic rates or establish experimental growth, thermodynamic feasibility, or flux identifiability. Setup and reproducibility Targets cobra 0.32.1 (import ), checked against its released source and current documentation. Plotting examples additionally require matplotlib, pandas, and seaborn. The extra supp…