HUMAnN Functional Profiler Skill Purpose Provide HUMAnN functional profiling for metagenomic pathway analysis. Capabilities - Gene family quantification - MetaCyc pathway abundance - UniRef annotation - Stratified functional profiles - Pathway coverage analysis - Custom database integration Usage Guidelines - Run taxonomic profiling first - Quantify gene families and pathways - Stratify by organism when relevant - Analyze pathway coverage - Compare functional profiles - Document database versions Dependencies - HUMAnN3 - eggNOG-mapper - Prokka Process Integration - Shotgun Metagenomics Pipeli…