PyDESeq2 Overview PyDESeq2 is a Python implementation of DESeq2 for differential expression analysis with bulk RNA-seq data. Design and execute complete workflows from data loading through result interpretation, including single-factor and multi-factor designs, Wald tests with multiple testing correction, optional apeGLM shrinkage, and integration with pandas and AnnData. When to Use This Skill This skill should be used when: - Analyzing bulk RNA-seq count data for differential expression - Comparing gene expression between experimental conditions (e.g., treated vs control) - Performing multi…