STAR RNA-seq Aligner Skill Purpose Provide STAR alignment for splice-aware RNA-seq read mapping with comprehensive QC metrics. Capabilities - Splice junction detection - Two-pass alignment mode - Chimeric read detection (fusions) - Gene quantification (--quantMode) - Custom genome index generation - Output in multiple formats Usage Guidelines - Generate genome indices with annotation - Use two-pass mode for novel junction discovery - Enable chimeric read detection for fusion analysis - Generate quantification in addition to alignments - Optimize parameters for read length - Document STAR vers…